TRANSBIO SUDOE aims at promoting interdisciplinary and interregional research in the field of health & life sciences.
TRANSBIO SUDOE can provide mobility support (transport & accommodation) for scientists interested in setting up collaborative research projects.
Below the procedure to launch a collaborative project :
Improved detection of Streptococcus pneumoniae from carriage samples using non-culturable methods
Request for technical support : - Need of TTO's support to ease collaboration agreements : No - Need for identification of funding opportunities (regional, national, European) : No
Added value of the cooperation : In the past we have already collaborated with Dr. Muñoz-Almagro through funding from Interbio, from which a paper was published (E. del Amo, P. Brotons, M. Monsonis, M. Triviño, M. Iñigo, L. Selva, R. Sá-Leão and C. Muñoz-Almagro (2013) High invasiveness of pneumococcal serotypes included in the new generation of conjugate vaccine. Clin Microbiol Infect. doi:10.1111/1469-0691.12422). With this new application we intend to continue this partnership. In the near future we are planning to submit joint proposals to the European Commission among other funding agencies.
Dr. Muñoz-Almagro has extensive experience in non-culturable methods to detect Streptococcus pneumoniae using clinical samples. Sónia Almeida will visit the laboratory of Dr. Muñoz-Almagro to learn how to optimize novel methodologies recently introduced at ITQB´s laboratory.
Summary of the proposal : Streptococcus pneumoniae(or pneumococcus) is a leading cause of infectious diseases worldwide. The incidence of invasive pneumococcal disease is age-dependent being highest at the extremes of age. However, while infection is a rare event in the lifecycle of this bacterium, colonization of the nasopharynx and oropharynx, is frequent and asymptomatic. Children are frequently colonized at high density by pneumococci in the first years of life, and the prevalence of carriage in this group is very high. Less is known about the carrier state among adults.
Currently, pneumococcal carriage is routinely detected by culture-based methods, with isolation of live pneumococci, which may result in a lower carriage prevalence estimate due to limited sensitivity of the method. Indeed, it has been reported that at a carriage density of ≤105 CFU/ml, the detection of pneumococci by culture may be compromised. More recently, new strategies to detect pneumococcal carriage based on real-time PCR have been developed. Studies have reported that the use of real-time PCR increased significantly the detection of carriage when compared to culture alone. Although molecular methods have superior sensitivity for bacterial detection, their specificity may be compromised due to difficulties in establishing species boundaries between pneumococcus and closely related species.The aim of this proposal is to optimize the detection of S. pneumoniae colonization through the use of a combination of culture and non-culturable methods.To accomplish this objective we will use nasopharyngeal and oropharyngeal DNA samples previously obtained from adults. The techniques are under implementation in the laboratory of Partner A. Sónia Almeida from Partner’s A laboratory will visit Partner B to receive know-how on how to optimize experimental procedures, avoid contamination problems and deal with odd results.
Ricardo RODRIGUEZ, Galicia Anais GONZALEZ, Barcelona
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